E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells (Record no. 61935)

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fixed length control field 01972nam a2200193Ia 4500
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control field MX-MdCICY
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20251009160707.0
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Transcribing agency CICY
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21845
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Title E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells
490 0# - SERIES STATEMENT
Series statement Nature Chemical Biology (2025). https://doi.org/10.1038/s41589-024-01809-9
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General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. The E2 ubiquitin (Ub)-conjugating enzyme primarily determines Ub conjugation as Ub-isopeptide (lysine), Ub-oxyester (serine/threonine) or Ub-thioester (cysteine). However, E2-specifc Ub conjugation profles within cells remain elusive. Here we developed the fusion E2-Ub-R74G profling (FUSEP) strategy to access E2-specifc Ub conjugation profles in cells with amino acid resolution. The probe-specifc leucine-glycine-glycine-glycine-modifed Ub remnant enables systematic studies of non-lysine Ub conjugation and provides site-specifc information. Multiple E2 enzymes were found to be involved in non-lysine ubiquitination. Profling with UBE2D3-Ub-R74G probes identifed a post-translational modifcation, tyrosine ubiquitination, in human Cullin-1, a scafold protein for Cullin-RING E3 Ub ligases. This modifcation is distinct from lysine ubiquitination. A single-pass membrane-bound E3 ligase, RNF149, was identifed to pair with UBE2D3 to regulate pyroptosis by ubiquitinating apoptosis-associated speck-like protein ASC. The availability of this toolbox paves the way for uncovering E2-specifc Ub conjugation profles and identifying previously unknown E3 Ub ligases for potential therapeutic applications.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element UB-R74G STRATEGY REVEALS E2-SPECIFIC UBIQUITIN CONJUGATION PROFILES IN LIVE CELLS
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Shen, S.;Yin, H.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1UuvGLdi9QQFjEUBO4h6uhSW2My8qFSa1/view?usp=drive_link">https://drive.google.com/file/d/1UuvGLdi9QQFjEUBO4h6uhSW2My8qFSa1/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 09.10.2025   B-21845 09.10.2025 09.10.2025 Documentos solicitados