Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca (Record no. 61944)

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control field MX-MdCICY
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control field 20251009160707.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21854
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Title Isolation and molecular characterization of the [Fe]-hydrogenase from the unicellular green alga Chlorella fusca
490 0# - SERIES STATEMENT
Series statement Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1576(3), p.330-334, 2002
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General note Artículo
520 3# - SUMMARY, ETC.
Summary, etc. [Fe]-hydrogenases are redoxenzymes that catalyze the reversible reduction of protons to hydrogen. Hydrogenase activity was observed in a culture of the unicellular green alga Chlorella fusca after an anaerobic incubation, but not in the related species Chlorella vulgaris. Specific polymerase chain reaction (PCR) techniques lead to the isolation of the cDNA and the genomic DNA of a special type of [Fe]-hydrogenase in C. fusca. The functional [Fe]-hydrogenase was purified to homogeneity and its N-terminus was sequenced. The polypeptide sequence shows a high degree of identity with the amino acid sequence deduced from the respective cDNA region. Structural and biochemical analyses indicate that ferredoxin is the main physiological electron donor. D 2002 Elsevier Science B.V. All rights reserved.
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CHLORELLA
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element [FE]-HYDROGENASE
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Topical term or geographic name entry element H2-EVOLUTION
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Topical term or geographic name entry element HYDROGEN
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element FERREDOXIN
700 12 - ADDED ENTRY--PERSONAL NAME
Personal name Winkler, M.;Heil, B.;Heil, B.;Happe, T.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1XI6CHoZ86gwJjEfHJnYL2mI7uBN-2W05/view?usp=drive_link">https://drive.google.com/file/d/1XI6CHoZ86gwJjEfHJnYL2mI7uBN-2W05/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 09.10.2025   B-21854 09.10.2025 09.10.2025 Documentos solicitados