MARC details
| 000 -LEADER |
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| fixed length control field |
02490nam a2200337Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20251009160708.0 |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) |
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| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
B-21876 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
251009s9999 xx 000 0 und d |
| 245 10 - TITLE STATEMENT |
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| Title |
De novo biosynthesis of carminic acid in Saccharomyces cerevisiae |
| 490 0# - SERIES STATEMENT |
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| Series statement |
Metabolic Engineering, 76, p.50-62, 2023 |
| 500 ## - GENERAL NOTE |
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| General note |
Artículo |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
Carminic acid is a natural red dye extracted from the insect Dactylopius coccus. Due to its ideal dying effect and high safety, it is widely used in food and cosmetics industries. Previous study showed that introduction of polyketide synthase (OKS) from Aloe arborescens, cyclase (ZhuI) and aromatase (ZhuJ) from Streptomyces sp. R1128, and C-glucosyltransferase (UGT2) from D. coccus into Aspergillus nidulans could achieve trace amounts of de novo production. These four genes were introduced into Saccharomyces cerevisiae, but carminic acid was not detected. Analysis of the genome of A. nidulans revealed that 4′-phosphopantetheinyl transferase (NpgA) and monooxygenase (AptC) are essential for de novo biosynthesis of carminic acid in S. cerevisiae. Additionally, endogenous hydroxylase (Cat5) from S. cerevisiae was found to be responsible for hydroxylation of flavokermesic acid to kermesic acid. Therefore, all enzymes and their functions in the biosynthesis of carminic acid were explored and reconstructed in S. cerevisiae. Through systematic pathway engineering, including regulating enzyme expression, enhancing precursor supply, and modifying the β-oxidation pathway, the carminic acid titer in a 5 L bioreactor reached 7580.9 μg/L, the highest yet reported for a microorganism. Heterologous reconstruction of the carminic acid biosynthetic pathway in S. cerevisiae has great potential for de novo biosynthesis of anthraquinone dye. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CARMINIC ACID IN SACCHAROMYCES CEREVISIAEMRE) |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
SACCHAROMYCES CEREVISIAE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
CARMINIC ACID |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
MALONYL-CoA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
ACETYL-CoA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
METABOLIC ENGINEERING |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
FATTY ACID B-OXIDATION |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhang, Q. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Wang, X. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zeng, W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Xu, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Li, D. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Yu, S. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Zhou, J. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="https://drive.google.com/file/d/1lqbmfutGfsoLDbLdcfUCWaGm7nlxtXGi/view?usp=drive_link">https://drive.google.com/file/d/1lqbmfutGfsoLDbLdcfUCWaGm7nlxtXGi/view?usp=drive_link</a> |
| Public note |
Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Clasificación local |
| Koha item type |
Documentos solicitados |
