Live long and prosper: acetate and its effects on longevity in batch culturing of Chlamydomonas reinhardtii. (Record no. 62019)
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| 000 -LEADER | |
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| fixed length control field | 02665nam a2200277Ia 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251009160709.0 |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | CICY |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | B-21929 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 251009s9999 xx 000 0 und d |
| 245 10 - TITLE STATEMENT | |
| Title | Live long and prosper: acetate and its effects on longevity in batch culturing of Chlamydomonas reinhardtii. |
| 490 0# - SERIES STATEMENT | |
| Series statement | Algal Research, 64, 102676, 2022. |
| 500 ## - GENERAL NOTE | |
| General note | Artículo |
| 520 3# - SUMMARY, ETC. | |
| Summary, etc. | The mass cultivation of algal biomass has several challenges, one of which is the poorly developed concepts of aging in batch-culture systems, that could ultimately affect biomass and bioproduct accumulation. While senescence and longevity are successfully applied to multicellular organisms, how these concepts apply to unicellular, photosynthetic eukaryotes is less developed. Chlamydomonas reinhardtii is a green mixotrophic microalga that can assimilate exogenous acetate as a carbon source as well as relying on photosynthesis for carbon fixation. We tested the hypothesis that caloric content in the media (acetate content) would influence longevity, as has been shown for animals and yeast. Here we describe the development of a culturing system to make studying senescence and aging more tractable while testing the effects of acetate on longevity. In our experiments, acetate had clear effects on biomass accumulation, chlorophyll content, starch accumulation, and cellular respiration. Higher levels of acetate also substantially decreased cell survival, observable in this system within 4-6 days. We also show that the high acetate-induced increase in starch levels was correlated with accelerated senescence and death. In support of this, mutants unable to synthesize starch do not show the same reduction in lifespan as the wildtype in high acetate cultures. Collectively, we hypothesize that the remodeling of metabolic flux under high acetate shortens lifespan, which may be due to the excessive redox state of the chloroplast and reactive oxygen generation that can follow. On the surface, this suggests that the concept of caloric restriction applies to photosynthetic mixotrophs and can influence longevity in batch culture systems. |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | MICROALGAL LONGEVITY |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CALORIC RESTRICTION |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CONDITIONAL SENESCENCE |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | CHLAMYDOMONAS REINHARDTII |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zamzam, G. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lee, C. W. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Milne, F. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Etsell, J. |
| 700 12 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | & Durnford, D. G. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://drive.google.com/file/d/1_-JX6zSWXTLEc6rp1iSUMSnBjPUXa7Ol/view?usp=drive_link">https://drive.google.com/file/d/1_-JX6zSWXTLEc6rp1iSUMSnBjPUXa7Ol/view?usp=drive_link</a> |
| Public note | Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Clasificación local |
| Koha item type | Documentos solicitados |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Clasificación local | Ref1 | CICY | CICY | Documento préstamo interbibliotecario | 09.10.2025 | B-21929 | 09.10.2025 | 09.10.2025 | Documentos solicitados |