Subgenome-informed statistical modeling of transcriptomes in 25 common wheat accessions reveals cis-and trans-regulation architectures. (Record no. 62046)

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control field MX-MdCICY
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control field 20251009160710.0
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Transcribing agency CICY
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Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) B-21958
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Title Subgenome-informed statistical modeling of transcriptomes in 25 common wheat accessions reveals cis-and trans-regulation architectures.
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Series statement Plant And Cell Physiology, 66(3), 347-357, 2025.
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General note Artículo
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Summary, etc. Common wheat is an allohexaploid, where it is difficult to obtain homoeolog-distinguished transcriptome data. Lasy-Seq, a type of 3ʹ RNA-seq, is technology efficient at obtaining homoeolog-distinguished transcriptomes. Here, we applied Lasy-Seq to obtain transcriptome data from the seedlings, second leaves, and root tips of 25 common wheat lines mainly from East Asia. Roots and seedlings were similar to each other in transcriptome profiles, but they were different from the leaves. We then asked how three homoeologous genes from different subgenomes (i.e. triads) show different levels of expression. Specifically, we examined the effects of subgenomes, lines, and their interaction on the expression levels of each homoeolog triad, separately in each tissue. Of the 19 805 homoeolog triads, 51-55% showed significant effect of subgenome, suggesting cis-regulation, whereas 24-30% showed significant effect line, suggesting trans-regulation. We also found that 7.7-9.0% triads showed significant effects of the interaction. Hierarchical clustering and co-trans regulation network analysis of homoeolog triads revealed that the patterns of expression polymorphisms among the lines were shared in different genes. Our results also implied that expression variation between lines is caused by changes in a smaller number of common trans-factors. We performed gene ontology (GO)-term enrichment analysis using newly annotated and substantially improved GO annotations, which revealed that GO terms related to each tissue-type function were enriched in genes expressed in the leaves and roots. Our information provides fundamental knowledge for the future breeding of plants possessing complex gene regulatory networks such as common wheat.
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Topical term or geographic name entry element COMMON WHEAT
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Topical term or geographic name entry element TRANSCRIPTOME
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Topical term or geographic name entry element LASY-SEQ
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element ALLOHEXAPLOID
650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element CIS-/TRANS-REGULATION
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Personal name Nomura, Y.
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Personal name Okada, M.
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Personal name Tameshige, T.
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Personal name Takenaka, S.
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Personal name Shimizu, K. K.
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Personal name Nasuda, S.
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Personal name Nagano, A. J.
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://drive.google.com/file/d/1U7p6hlgavIu35ycUn1TIjf_Ow7qV9a1c/view?usp=drive_link">https://drive.google.com/file/d/1U7p6hlgavIu35ycUn1TIjf_Ow7qV9a1c/view?usp=drive_link</a>
Public note Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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Source of classification or shelving scheme Clasificación local
Koha item type Documentos solicitados
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  Clasificación local     Ref1 CICY CICY Documento préstamo interbibliotecario 09.10.2025   B-21958 09.10.2025 09.10.2025 Documentos solicitados