MARC details
| 000 -LEADER |
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| fixed length control field |
02982nam a2200313Ia 4500 |
| 001 - CONTROL NUMBER |
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| control field |
000008275 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
MX-MdCICY |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20260112162205.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
130617s2014 nyua f p 0|1 | eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9781621820789 (cloth) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9781936113583 (pbk) |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CICY |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
C 572.516 |
| Item number |
C35 2014 |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Calcium techniques : |
| Remainder of title |
a laboratory manual / |
| Statement of responsibility, etc. |
[edited by] Jan B. Parys, Martin D. Bootman, David I. Yule, Geert Bultynck |
| 264 31 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE |
|---|
| Place of production, publication, distribution, manufacture |
New York : |
| Name of producer, publisher, distributor, manufacturer |
Cold Spring Harbor Laboratory Press, |
| Date of production, publication, distribution, manufacture, or copyright notice |
c2014 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xvi, 592 p. : |
| Other physical details |
il. ; |
| Dimensions |
28 cm. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Incluye referencias bibliográficas e índice |
| 520 3# - SUMMARY, ETC. |
|---|
| Summary, etc. |
Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to ra úsele contraction and nerve impulses. To créate spatiotemporally distinct Ca2+ signáis, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principies and applica-tions of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imag-ing modalities to characterize Ca2+ signáis. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and meth-ods to investígate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and inter-cellular signáis are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in nonmammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CALCIO, CANALES |
| Form subdivision |
MANUALES DE LABORATORIO |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CALCIO, SEÑALES |
| Form subdivision |
MANUALES DE LABORATORIO |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
CALCIO |
| Form subdivision |
MANUALES DE LABORATORIO |
| General subdivision |
FISIOLOGIA |
| 650 14 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name entry element |
FLUOROMETRIA |
| Form subdivision |
MANUALES DE LABORATORIO |
| General subdivision |
METODOS |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bootman, Martin D., |
| Relator term |
ed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Bultynck, Geert, |
| Relator term |
ed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Parys, Jan B., |
| Dates associated with a name |
1958-, |
| Relator term |
ed. |
| 700 12 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Yule, David I., |
| Dates associated with a name |
1964-, |
| Relator term |
ed. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
|---|
| Uniform Resource Identifier |
<a href="https://www.cicy.mx/sitios/sib/doctoelectronico/8275.pdf">https://www.cicy.mx/sitios/sib/doctoelectronico/8275.pdf</a> |
| Public note |
Ver tabla de contenido y/o resumen |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Libros impresos |
