Biocontrol activity of Trichoderma harzianum, Bacillus subtilis and Pseudomonas fluorescens against Meloidogyne incognita, Fusarium oxysporum and Rhizoctonia solani

Artículo

Repeated in-vitro trials were conducted to study the biocontrol potential of Trichoderma harzianum, Bacillus subtilis, and Pseudomonas fuorescens against Meloidogyne incognita, Fusarium oxysporum and Rhizoctonia solani. The interaction studies of biocontrol agents and pathogens showed that undiluted culture fltrates of T. harzianum, B. subtilis and P. fuorescens caused 100 percent mortality against M. incognita and inhibit its egg hatching up to 75 percent. M. incognita eggs were also found infected (up to 89 percent) when exposed to fresh culture of T. harzianum. Tested biocontrol agents caused growth inhibi[1]tion against F. oxysporum and R. solani when exposed to dual culture bioassays. T. harzianum caused maximum growth inhibition against F. oxysporum (66 percent) and R. solani (99 percent) followed by P. fuorescens (40 and 83 percent) and B. subtilis (41 and 48 percent) respectively. T. harzianum, B. subtilis and P. fuorescens showed compatibility among them under in-vitro trials and did not show any negative impact on each other. In contrast, the interaction of biocontrol agents with all tested pathogens was recorded negative or antagonistic. Our fndings proved that compatibility and biocontrol potential of T. harzianum, B. subtilis and P. fuorescens makes them a potential tool to control soil borne pathogens (M. incognita, F. oxysporum and R. solani), which may replace harmful and costly management options and could be an important component of IPM strategies for sustainable agriculture production