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Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes

Tipo de material: TextoTextoSeries ; Journal of Biotechnology, 16(3-4), p.171-186, 1990Trabajos contenidos:
  • Sharp, J.M
  • Doran, P.M
Tema(s): Recursos en línea: Resumen: Growth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The specific growth rate was 0.28 d-1, corresponding to a doubling time of 60 h. In airlift reactors, first order kinetics were not observed due to nutrient limitation. Synthesis of tropane alkaloids was growth-associated; no atropine was released into the medium. Sugar concentrations were analyzed during hairy-root culture for calculation of atropine yield. Atropine yields from substrate were 1.4 mg g-1 in shake flasks and 0.46 mg g-1 in airlift reactors. Although levels of atropine in reactor-grown roots (0.37
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-6047 (Browse shelf(Opens below)) Available

Growth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The specific growth rate was 0.28 d-1, corresponding to a doubling time of 60 h. In airlift reactors, first order kinetics were not observed due to nutrient limitation. Synthesis of tropane alkaloids was growth-associated; no atropine was released into the medium. Sugar concentrations were analyzed during hairy-root culture for calculation of atropine yield. Atropine yields from substrate were 1.4 mg g-1 in shake flasks and 0.46 mg g-1 in airlift reactors. Although levels of atropine in reactor-grown roots (0.37

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