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Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi

Tipo de material: TextoTextoSeries ; MicroBiology, 147(5), p.1171-1182, 2001Trabajos contenidos:
  • Arnold, D.L
  • Jackson, R.W
  • Fillingham, A.J
  • Goss, S.C
  • Taylor, J.D
  • Mansfield, J.W
  • Vivian, A
Tema(s): Recursos en línea: Resumen: DNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1)from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with all P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- and avrPpiB-containing fragments, two additional fragments were amplified: one contained a single open reading frame (ORF1)and was found in races of genomic group II (2, 3A, 4A and 6); the second fragment contained two open reading frames (ORF2 and ORF3), separated by 658 nt, and was detected in all races. All three ORFs had G+C ratios (46·9-48 mol
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-6270 (Browse shelf(Opens below)) Available

DNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1)from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with all P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- and avrPpiB-containing fragments, two additional fragments were amplified: one contained a single open reading frame (ORF1)and was found in races of genomic group II (2, 3A, 4A and 6); the second fragment contained two open reading frames (ORF2 and ORF3), separated by 658 nt, and was detected in all races. All three ORFs had G+C ratios (46·9-48 mol

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