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An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and Red

Tipo de material: TextoTextoSeries ; FEMS MicroBiology Letters , 234(2), p.325-331 , 2004Trabajos contenidos:
  • Fukiya, S
  • Mizoguchi, H
  • Mori, H
Tema(s): Recursos en línea: Resumen: We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by Red and large deletions of both 117- and 165-kbp regions could be generated in 100
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We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by Red and large deletions of both 117- and 165-kbp regions could be generated in 100

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