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Detection of pectinesterase in polyacrylamide gels.

Tipo de material: TextoTextoSeries ; Electrophoresis, 16(1), p.39-42., 1995Trabajos contenidos:
  • Alonso, J
  • Rodriguez, MT
  • Canet, W
Recursos en línea: Resumen: A rapid and sensitive method of detecting pectinesterase activity following electrophoresis or isoelectric focusing in polyacrylamide gels is described. The method uses ruthenium red and requires no addition of substrate when making the gels, thus obviating direct enzyme-substrate contact during electrophoresis. Because of its versatility, the method can be used in a wide variety of applications, such as plant and microbial taxonomy, enzyme purification and characterization, or as an analytical method in fresh and processed plant technology
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-6588 (Browse shelf(Opens below)) Available

A rapid and sensitive method of detecting pectinesterase activity following electrophoresis or isoelectric focusing in polyacrylamide gels is described. The method uses ruthenium red and requires no addition of substrate when making the gels, thus obviating direct enzyme-substrate contact during electrophoresis. Because of its versatility, the method can be used in a wide variety of applications, such as plant and microbial taxonomy, enzyme purification and characterization, or as an analytical method in fresh and processed plant technology

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