Absorption of immunomodulating beta(1,3)-glucan in yolk sac larvae of Atlantic halibut, Hippoglossus hippoglossus (L.)

Tipo de material: Texto

TextoSeries ; Journal of Fish Diseases, 20(1), p.41-49, 1997Trabajos contenidos:

Strand, H.K
Dalmo, R.A

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Resumen: A soluble immunomodulating beta(1,3)-glucan, laminaran, was given in drinking water to yolk-sac larvae of Atlantic halibut, Hippoglossus hippoglossus L. The larvae absorbed laminaran when the concentration was 25 mg l-1. After 5 days exposure to laminaran, each larva contained 2.3 ng and 46 ng of 3H- and 125I-labelled laminaran, respectively. After 10 days exposure, each larva contained 4.5 ng and 47 ng of 3H- and 125I-laminaran, respectively. The absorption was confirmed by fluorescence microscopy of larvae exposed to fluorescein-labelled laminaran (FITC-laminaran). The fluorescence was localized to cells in the intestinal epithelial layer and the skin epithelial layer.

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A soluble immunomodulating beta(1,3)-glucan, laminaran, was given in drinking water to yolk-sac larvae of Atlantic halibut, Hippoglossus hippoglossus L. The larvae absorbed laminaran when the concentration was 25 mg l-1. After 5 days exposure to laminaran, each larva contained 2.3 ng and 46 ng of 3H- and 125I-labelled laminaran, respectively. After 10 days exposure, each larva contained 4.5 ng and 47 ng of 3H- and 125I-laminaran, respectively. The absorption was confirmed by fluorescence microscopy of larvae exposed to fluorescein-labelled laminaran (FITC-laminaran). The fluorescence was localized to cells in the intestinal epithelial layer and the skin epithelial layer.

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