Purification of the plant alternative oxidase from Arum maculatum: measurement, stability and metal requirement
Tipo de material:
TextoSeries ; Biochimica et Biophysica Acta (BBA)- Bioenergetics , 1608(2-3), p.181-189, 2004Trabajos contenidos: - Affourtit, C
- Moore. A.L
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We have purified plant alternative oxidase (AOX)protein from the spadices of thermogenic Arum maculatum (cuckoo pint)to virtual homogeneity. The obtained enzyme fraction exhibits a high specific activity, consuming on average 32 µmol oxygen min-1 mg-1, which is completely stable for at least 6 months when the sample is stored at -70 °C. This exceptionally stable AOX activity is inhibited approximately 90
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