The genes encoding granule-bound starch synthases at the waxy loci of the A, B, and D progenitors of common wheat
Tipo de material:
TextoSeries ; Genome, 43(2), p.264-272, 2000Trabajos contenidos: - Yan, L
- Bhave, M
- Fairclough, R
- Konik, Ch
- Rahman, S
- Appels, R
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Three genes encoding granule-bound starch synthase (wx-TmA, wx-TsB, and wx-TtD)have been isolated from Triticum monococcum (AA), and Triticum speltoides (BB), by the polymerase chain reaction (PCR)approach, and from Triticum tauschii (DD), by screening a genomic DNA library. Multiple sequence alignment indicated that the wx-TmA, wx-TsB, and wx-TtD genes had the same extron and (or)intron structure as the previously reported waxy gene from barley. The lengths of the three wx-TmA, wx-TsB, and wx-TtD genes were 2834 bp, 2826 bp, and 2893 bp, respectively, each covering 31 bp in the untranslated leader and the entire coding region consisting of 11 exons and 10 introns. The three genes had identical lengths of exons, except exon1, and shared over 95
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