Cloning, sequencing and salt induced expression of PEAMTand BADH in oilseed rape (Brassica napus)

Tipo de material: Texto

TextoSeries ; DNA Sequence, 16(5), p.364-371, 2005Trabajos contenidos:

Ye, C
Wu, S
Yang, Q
Ma, C
Yang, G
Wang, B

Tema(s):

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Resumen: Agriculture productivity is severely hampered by soil salinity, drought and other environmental stresses. Studies on stressresistant plants (halophytes, xerophytes, accumulating plants for specific toxic ions)have illuminated some mechanisms of stress tolerance in plants at metabolic or molecular levels, which gave some clues on how to genetically engineer stress-tolerant crops. With the isolation of more stress-responsive genes, genetic engineering with modified expression of stress responsive genes may be an effective way to produce stress-tolerant crops. In the present report, two genes (PEAMT and BADH)encoding the corresponding key enzymes for choline and glycine betaine (an important osmoprotectant)biosynthesis in plants were isolated in oilseed rape, an important oil crop in the world. Effects of salt stress on their expression were studied with quantitative PCR and their potential use in the genetic engineering of oilseed rape was discussed.

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Agriculture productivity is severely hampered by soil salinity, drought and other environmental stresses. Studies on stressresistant plants (halophytes, xerophytes, accumulating plants for specific toxic ions)have illuminated some mechanisms of stress tolerance in plants at metabolic or molecular levels, which gave some clues on how to genetically engineer stress-tolerant crops. With the isolation of more stress-responsive genes, genetic engineering with modified expression of stress responsive genes may be an effective way to produce stress-tolerant crops. In the present report, two genes (PEAMT and BADH)encoding the corresponding key enzymes for choline and glycine betaine (an important osmoprotectant)biosynthesis in plants were isolated in oilseed rape, an important oil crop in the world. Effects of salt stress on their expression were studied with quantitative PCR and their potential use in the genetic engineering of oilseed rape was discussed.

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