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Direct shoot organogenesis on shoot apex from seedling explants of Capsicum annuum L.

Tipo de material: TextoTextoSeries ; Scientia Horticulturae, 106(2), p.237, 2005Trabajos contenidos:
  • Kumar, V
  • Gururaj, H.B
  • Narasimha Prasad, B.C
  • Giridhar, P
  • Ravishankar, G.A
Tema(s): Recursos en línea: Resumen: In vitro direct multiple shoot formation from seedling explants of Indian high pungent varieties of Capsicum annuum cv. Arka Abhir (AA)and Arka Lohit (AL)was successfully obtained. We were able to induce regeneration potency in these varieties by inverting the explant. Aseptically grown seedling explants with decapitated roots, apical meristem and cotyledonary leaves were inoculated in an inverted position in bud induction medium comprising of Murashige and Skoog's basal medium supplemented with 2-(N-morpholine)ethanesulphonic acid (MES)buffer along with 26.63 mM benzyl adenine (BA), 2.28 mM indole-3-acetic acid (IAA)and 10 mM silver nitrate. Profuse shoot bud induction with 20-25 shoot buds per explant was obtained. Supplementation of phloroglucinol in the bud induction medium resulted in 17 and 18
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In vitro direct multiple shoot formation from seedling explants of Indian high pungent varieties of Capsicum annuum cv. Arka Abhir (AA)and Arka Lohit (AL)was successfully obtained. We were able to induce regeneration potency in these varieties by inverting the explant. Aseptically grown seedling explants with decapitated roots, apical meristem and cotyledonary leaves were inoculated in an inverted position in bud induction medium comprising of Murashige and Skoog's basal medium supplemented with 2-(N-morpholine)ethanesulphonic acid (MES)buffer along with 26.63 mM benzyl adenine (BA), 2.28 mM indole-3-acetic acid (IAA)and 10 mM silver nitrate. Profuse shoot bud induction with 20-25 shoot buds per explant was obtained. Supplementation of phloroglucinol in the bud induction medium resulted in 17 and 18

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