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Identi¢cation of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants

Tipo de material: TextoTextoSeries ; FEBS Letters, 437, p.56-60, 1998Trabajos contenidos:
  • Mizoguchi, T
  • Ichimura, K
  • Irie, K
  • Morris, P
  • Giraudat, J
  • Matsumoto, K
  • Shinozaki, K
Tema(s): Recursos en línea: Resumen: A possible MAP kinase (MAPK)cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK)and MEK1 (a MAPKK)and interactions between MEK1 and ATMEKK1 (a MAPKKK)were detected by using the 2-hybrid system. A growth defect of the yeast mpk1v mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2v mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants.
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A possible MAP kinase (MAPK)cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK)and MEK1 (a MAPKK)and interactions between MEK1 and ATMEKK1 (a MAPKKK)were detected by using the 2-hybrid system. A growth defect of the yeast mpk1v mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2v mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants.

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