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Production and Secretion of Indole Alkaloids in Hairy Root Cultures of Catharanthus roseus: Effects of In Situ Adsorption, Fungal Elicitation and Permeabilization

Tipo de material: TextoTextoSeries ; Journal of Fermentation and BioEngineering, 78(3), p.229-234, 1994Trabajos contenidos:
  • Sim, S.J
  • Chang, H.N
  • Liu, J.R
  • Hung, K.H
Recursos en línea: Resumen: Various hairy root clones were derived by transforming two kinds of Catharanthus roseus plants (Catharanthus roseus cvs. Little Linda and Little Delicata)with Agrobacterium rhizogenes ATCC 15834. Hairy root growth and indole alkaloid production were then investigated to select a high-yielding hairy root clone. Among three Amberlite resins (XAD-2, XAD-4, and XAD-7), XAD-7 greatly enhanced the release of catharanthine and ajmalicine from hairy root cultures, with an increase in total production. To enhance alkaloid production and secretion, a permeabilizing agent (dimethyl sulfoxide)and a fungal elicitor to provide physical and biochemical stress, respectively, together with in situ adsorption. Dimethyl sulfoxide (0.5
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Various hairy root clones were derived by transforming two kinds of Catharanthus roseus plants (Catharanthus roseus cvs. Little Linda and Little Delicata)with Agrobacterium rhizogenes ATCC 15834. Hairy root growth and indole alkaloid production were then investigated to select a high-yielding hairy root clone. Among three Amberlite resins (XAD-2, XAD-4, and XAD-7), XAD-7 greatly enhanced the release of catharanthine and ajmalicine from hairy root cultures, with an increase in total production. To enhance alkaloid production and secretion, a permeabilizing agent (dimethyl sulfoxide)and a fungal elicitor to provide physical and biochemical stress, respectively, together with in situ adsorption. Dimethyl sulfoxide (0.5

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