Cloning and Expression of Papaya Ring Spot Virus (PRSV)Coat Protein Gene in Bacteria

Tipo de material: Texto

TextoSeries ; Acta Horticulturae, 740, p.141-146, 2007Trabajos contenidos:

Tan Chon-Seng
Tan Poh Hwa
Ong Ching Ang
Hassan Mat Daud

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Resumen: The papaya ring-spot virus (PRSV)coat protein gene was obtained by RT-PCR amplification of viral RNA isolated directly from virus-infected papaya leaf. The PCR amplified fragment was then ligated into cloning vector for gene verification and sequencing. The DNA sequencing result showed that the cloned PRSV coat protein gene was 927bp in size. The PRSV nucleotide and predicted amino acid sequence showing more than 95

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The papaya ring-spot virus (PRSV)coat protein gene was obtained by RT-PCR amplification of viral RNA isolated directly from virus-infected papaya leaf. The PCR amplified fragment was then ligated into cloning vector for gene verification and sequencing. The DNA sequencing result showed that the cloned PRSV coat protein gene was 927bp in size. The PRSV nucleotide and predicted amino acid sequence showing more than 95

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