Within-tree transcriptome profiling in wood-forming tissues of a fast-growing Eucalyptus tree

Tipo de material: Texto

TextoSeries ; Tree Physiology, 26(3), p.365-375, 2006Trabajos contenidos:

Ranik, M
Creux, N.M
Myburg, A.A

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Resumen: Despite the availability of high-throughput transcript profiling technology, little is known about tissue-specific gene expression patterns in the wood-forming tissues of Eucalyptus plantation tree species.We used cDNA-amplified fragment length polymorphism (AFLP)analysis in combination with infrared fragment detection and semi-automated band quantification to profile gene expression in a 6-year-old, fastgrowing Eucalyptus tree. The expression profiles of 6385 transcript- derived fragments (TDFs)were analyzed across four major woody tissues (mature xylem, immature xylem, phloem and cork)collected from two stem positions, to provide a global view of transcript abundance and variability in the Eucalyptus stem. About 21

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Despite the availability of high-throughput transcript profiling technology, little is known about tissue-specific gene expression patterns in the wood-forming tissues of Eucalyptus plantation tree species.We used cDNA-amplified fragment length polymorphism (AFLP)analysis in combination with infrared fragment detection and semi-automated band quantification to profile gene expression in a 6-year-old, fastgrowing Eucalyptus tree. The expression profiles of 6385 transcript- derived fragments (TDFs)were analyzed across four major woody tissues (mature xylem, immature xylem, phloem and cork)collected from two stem positions, to provide a global view of transcript abundance and variability in the Eucalyptus stem. About 21

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