Comparison of growth properties of carrot hairy root in various bioreactors

Tipo de material: Texto

TextoSeries ; Appl Microbiol Biotechnol, 32, p.291-294, 1989Trabajos contenidos:

Kondo, O
Honda, H
Taya, M
Kobayashi, T

Recursos en línea:

Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx

Resumen: Growth properties of carrot hairy root cells in various bioreactors were investigated. A turbine-blade reactor and an immobilized rotating drum reactor were found to be advantageous for the hairy root culture because of a high oxygen transfer coefficient (kLa). After 30 days of culture, 10 g/1 of dry hairy root cells were obtained in both bioreactors and maximum growth rates (Vm)were found to be 0.63 and 0.61 g/1 per day for the turbine-blade reactor and immobilized rotating drum reactor, respectively. Specific growth rates ~)at various cultivation times were observed to be linearly proportional to X/kLa for both bioreactor configurations where X is the cell concentration. The estimated specific oxygen uptake rate of 0.34 mmol O2/g dry cells per hour compares fairly well with an experimental value of 0.3.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Documentos solicitados	CICY Documento préstamo interbibliotecario	Ref1	B-10316 (Browse shelf(Opens below))	Available

Browsing CICY shelves, Shelving location: Documento préstamo interbibliotecario, Collection: Ref1 Close shelf browser (Hides shelf browser)

Previous	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	No cover image available	Next
Previous	B-10313 A new method for the production of fine plant cell suspension cultures	B-10314 Callus and suspension culture of Lupinus angustifolius cv. Turkus	B-10315 Osmolarity as a measure of growth of plant cells in suspension cultures	B-10316 Comparison of growth properties of carrot hairy root in various bioreactors	B-10317 El macuilillo	B-10318 Feeding hungry plants: The role of purple acid phosphatases in phosphate nutrition	B-10319 Conservation status of wild plams in Latin America and the Caribbean	Next

Growth properties of carrot hairy root cells in various bioreactors were investigated. A turbine-blade reactor and an immobilized rotating drum reactor were found to be advantageous for the hairy root culture because of a high oxygen transfer coefficient (kLa). After 30 days of culture, 10 g/1 of dry hairy root cells were obtained in both bioreactors and maximum growth rates (Vm)were found to be 0.63 and 0.61 g/1 per day for the turbine-blade reactor and immobilized rotating drum reactor, respectively. Specific growth rates ~)at various cultivation times were observed to be linearly proportional to X/kLa for both bioreactor configurations where X is the cell concentration. The estimated specific oxygen uptake rate of 0.34 mmol O2/g dry cells per hour compares fairly well with an experimental value of 0.3.

There are no comments on this title.

to post a comment.