Identification of a new laccase gene and confirmation of genomic predictions by cDNA sequences of Trametes sp. I-62 laccase family

Tipo de material: Texto

TextoSeries ; Mycological Research, 107(6), p.727-735, 2003Trabajos contenidos:

González, T
Terrón, M.C
Zapico, E
Yague S
Téllez, A
Junca, H
González, A

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Resumen: The strain Trametes sp. I-62 (CECT 20197)is a white-rot fungus with great potential for biotechnological applications in the fields of industrial waste water decolorization and clean up. Three laccase genes: lcc1, lcc2 and lcc3 have been cloned and sequenced from this basidiomycete. In this work, the coding regions of the corresponding cDNAs have been synthesized, cloned, and sequenced. They are 1563, 1563 and 1575 bp in length, respectively. Former putative intron/ exon structures from genomic DNA are fully confirmed by match analysis with our cDNA sequences. Using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP)analysis, an additional laccase cDNA was also identified, corresponding to a new gene, lcc1A, which displayed 99.6

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The strain Trametes sp. I-62 (CECT 20197)is a white-rot fungus with great potential for biotechnological applications in the fields of industrial waste water decolorization and clean up. Three laccase genes: lcc1, lcc2 and lcc3 have been cloned and sequenced from this basidiomycete. In this work, the coding regions of the corresponding cDNAs have been synthesized, cloned, and sequenced. They are 1563, 1563 and 1575 bp in length, respectively. Former putative intron/ exon structures from genomic DNA are fully confirmed by match analysis with our cDNA sequences. Using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP)analysis, an additional laccase cDNA was also identified, corresponding to a new gene, lcc1A, which displayed 99.6

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