Manipulation of Microalgal Lipid Production Using Genetic Engineering
Tipo de material:
TextoSeries ; Applied BioChemistry and Biotechnology, 57/58, p.223-231, 1996Trabajos contenidos: - Dunahay, T.G
- Jarvls, E.E
- Dais, S.S
- Roessler, P.G
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Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase)gene from the diatom Cyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was pro- duced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to mani- pulate lipid accumulation in transformed strains.
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