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HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA)glucose ester, and linkage of pHBA into cell walls

Tipo de material: TextoTextoSeries ; Bioresource Technology, 100(20), p.4836-4842, 2009Trabajos contenidos:
  • Rahman, L
  • Kouno, H
  • Hashiguchi, Y
  • Yamamoto, H
  • Narbad, A
  • Parr, A
  • Walton, N
  • Walton, N
  • Kitamura, Y
Tema(s): Recursos en línea: Resumen: As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet)has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA)at about 14
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-11022 (Browse shelf(Opens below)) Available

As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet)has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase, was introduced into B. vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analysed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA)at about 14

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