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A highly sensitive technique for staining DNA and RNA in polyacrylamide gels using silver

Tipo de material: TextoTextoSeries ; Journal of BioChemical and Biophysical Methods, 4, p.219-228, 1981Trabajos contenidos:
  • Boulikas, T
  • Hancock, R
Tema(s): Recursos en línea: Resumen: A technique is described for staining DNA in polyacr?,lamide gels with sdver. It is rapid, requiring about 30 rain for the whole stmning and development procedure, ve~' smlple and at least 20 times more sensitive than ethidium bromide for the staining of double-stranded DNA an polyacD,lamide gels. This techmque can also be applied for the stmning of denatured, single-stranded DNA as well as RNA after their electrophoretic separation on polyac~'lamide gels, having the same sensitivity as for double-stranded DNA fragments.
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A technique is described for staining DNA in polyacr?,lamide gels with sdver. It is rapid, requiring about 30 rain for the whole stmning and development procedure, ve~' smlple and at least 20 times more sensitive than ethidium bromide for the staining of double-stranded DNA an polyacD,lamide gels. This techmque can also be applied for the stmning of denatured, single-stranded DNA as well as RNA after their electrophoretic separation on polyac~'lamide gels, having the same sensitivity as for double-stranded DNA fragments.

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