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A novel approach for extraction of PCR-compatible DNA from activated sludge samples collected from different biological effluent treatment plants

Tipo de material: TextoTextoSeries ; Journal of MicroBiological Methods, 52(3), p.315-323, 2003Trabajos contenidos:
  • Purohit, H.J
  • Kapley, A
  • Moharikar, A.A
  • Narde, G
Tema(s): Recursos en línea: Resumen: This paper describes a method that facilitates the extraction of PCR-compatible DNA from different activated sludge samples. The approach involves a novel preprocessing step in DNA extraction, which removes potential PCR inhibitors. The sludge was washed with different ratios of acetone and petroleum ether after pretreatment with 0.01
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This paper describes a method that facilitates the extraction of PCR-compatible DNA from different activated sludge samples. The approach involves a novel preprocessing step in DNA extraction, which removes potential PCR inhibitors. The sludge was washed with different ratios of acetone and petroleum ether after pretreatment with 0.01

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