dsRNA-induced gene silencing in Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao

Tipo de material: Texto

TextoSeries ; Fungal genetics and Biology, 46(11), p.825-836, 2009Trabajos contenidos:

Caribé Dos Santos A.C
Sena, J.A
Santos, S.C
Dias, C.V
Pirovani, C.P
Pungartnik, C
Valle, R.R
Valle, R.R
Vincent, M

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Resumen: The genome sequence of the hemibiotrophic fungus Moniliophthora perniciosa revealed genes possibly participating in the RNAi machinery. Therefore, studies were performed in order to investigate the efficiency of gene silencing by dsRNA. We showed that the reporter gfp gene stably introduced into the fungus genome can be silenced by transfection of in vitro synthesized gfpdsRNA. In addition, successful dsRNA-induced silencing of endogenous genes coding for hydrophobins and a peroxiredoxin were also achieved. All genes showed a silencing efficiency ranging from 18

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The genome sequence of the hemibiotrophic fungus Moniliophthora perniciosa revealed genes possibly participating in the RNAi machinery. Therefore, studies were performed in order to investigate the efficiency of gene silencing by dsRNA. We showed that the reporter gfp gene stably introduced into the fungus genome can be silenced by transfection of in vitro synthesized gfpdsRNA. In addition, successful dsRNA-induced silencing of endogenous genes coding for hydrophobins and a peroxiredoxin were also achieved. All genes showed a silencing efficiency ranging from 18

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