Deep sequencing analysis of viruses infecting grapevines: Virome of a vineyard
Tipo de material:
TextoSeries ; Virology, 400(2), p.157-163, 2010Trabajos contenidos: - Coetzee, B
- Freeborough, M.J
- Maree, H.J
- Celton, J.M
- Jasper, D
- Rees, G
- Burger, J.T
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Double stranded RNA, isolated from 44 pooled randomly selected vines from a diseased South African vineyard, has been used in a deep sequencing analysis to build a census of the viral population. The dsRNA was sequenced in an unbiased manner using the sequencing-by-synthesis technology offered by the Illumina Genome Analyzer II and yielded 837 megabases of metagenomic sequence data. Four known viral athogens were identified. It was found that Grapevine leafroll-associated virus 3 (GLRaV-3)is the most prevalent species, constituting 59
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