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GATEWAY vectors for Agrobacterium-mediated plant transformation

Tipo de material: TextoTextoSeries ; Trends in Plant Science, 7(5), p.193-195, 2002Trabajos contenidos:
  • Karimi, M
  • Inzé, D
  • Depicker, A
Recursos en línea: Resumen: Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAYÆËconversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.
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Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAYÆËconversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.

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