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Simultaneous measurement of proline and related compounds in oak leaves by high-performance ligand-exchange chromatography and electrospray ionization mass spectrometry for environmental stress studies

Tipo de material: TextoTextoSeries ; Journal of Chromatography A, 1216, p.1094-1099, 2009Trabajos contenidos:
  • Oufira, M
  • Schulz, N
  • Sha Vallikhan, P.S
  • Wilhelm, E
  • Burg, K
  • Hausman, J
  • Hoffmann, L
  • Hoffmann, L
Recursos en línea: Resumen: A mass spectrometerwas coupled to high-performance ligand-exchange liquid chromatography (HPLEC)for simultaneous analysis of stress associated solutes such as proline, hydroxyproline, methylproline, glycine betaine and trigonelline extracted from leaves of drought stressed oaks and an internal standard namely N-acetylproline. Methanol/chloroform/water extracts were analyzed using an Aminex HPX-87C column and specifically quantified by the positive ion mode of an electrospray ionisation-mass spectrometry (ESI-MS)in single ion monitoring (SIM)mode. The recovery of N-acetyl proline added to oak leaf extracts ranged from 85.2 to 122.1
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-12048 (Browse shelf(Opens below)) Available

A mass spectrometerwas coupled to high-performance ligand-exchange liquid chromatography (HPLEC)for simultaneous analysis of stress associated solutes such as proline, hydroxyproline, methylproline, glycine betaine and trigonelline extracted from leaves of drought stressed oaks and an internal standard namely N-acetylproline. Methanol/chloroform/water extracts were analyzed using an Aminex HPX-87C column and specifically quantified by the positive ion mode of an electrospray ionisation-mass spectrometry (ESI-MS)in single ion monitoring (SIM)mode. The recovery of N-acetyl proline added to oak leaf extracts ranged from 85.2 to 122.1

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