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Identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2)gene from the microalga O. tauri

Tipo de material: TextoTextoSeries ; Plant Physiology and BioChemistry, 48(6), p.407-416, 2010Trabajos contenidos:
  • Wagner, M
  • Hoppe, K
  • Czabany, T
  • Heilmann, M
  • Daum, G
  • Feussner, I
  • Fulda, M
Tema(s): Recursos en línea: Resumen: In order to identify novel genes encoding enzymes involved in the terminal step of triacylglycerol (TAG)formation, a database search was carried out in the genome of the unicellular photoautotrophic green alga Ostreococcus tauri. The search led to the identification of three putative type 2 acyl-CoA:diacylglycerol acyltransferase-like sequences (DGAT; EC 2.3.1.20), and revealed the absence of any homolog to type 1 or type 3 DGAT sequence in the genome of O. tauri. For two of the cDNA sequences (OtDGAT2A and B)enzyme activity was detected by heterologous expression in Saccharomyces cerevisiae mutant strains with impaired TAG metabolism. However, activity of OtDGAT2A was too low for further analysis. Analysis of their amino acid sequences showed that they share limited identity with other DGAT2 from different plant species, such as Ricinus communis and Vernicia fordii with w25 to 30
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In order to identify novel genes encoding enzymes involved in the terminal step of triacylglycerol (TAG)formation, a database search was carried out in the genome of the unicellular photoautotrophic green alga Ostreococcus tauri. The search led to the identification of three putative type 2 acyl-CoA:diacylglycerol acyltransferase-like sequences (DGAT; EC 2.3.1.20), and revealed the absence of any homolog to type 1 or type 3 DGAT sequence in the genome of O. tauri. For two of the cDNA sequences (OtDGAT2A and B)enzyme activity was detected by heterologous expression in Saccharomyces cerevisiae mutant strains with impaired TAG metabolism. However, activity of OtDGAT2A was too low for further analysis. Analysis of their amino acid sequences showed that they share limited identity with other DGAT2 from different plant species, such as Ricinus communis and Vernicia fordii with w25 to 30

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