A Minimum Estimate for the Length of the DNA of Escherichia Coli obtained by Autoradiography

Tipo de material: Texto

TextoSeries ; J. Mol. Biol., 4(5), p.407-409, 1962Trabajos contenidos:

Cairns, J

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Resumen: E. coli labelled with [3H]thymine was lysed in EDTA and 2 M-sucrose by dialysis against Duponol. After 20 hours the DNA of some bacteria had partly untangled and was trapped on the wall ofthe dialysis membrane when the dialysis chamber was drained. Autoradiography showed this DNA to be several hundred microns long, indicating a molecular weight of 109 or more. Short fragments, which would correspond to the usual molecular weight of extracted bacterial DNA, were not seen.

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E. coli labelled with [3H]thymine was lysed in EDTA and 2 M-sucrose by dialysis against Duponol. After 20 hours the DNA of some bacteria had partly untangled and was trapped on the wall ofthe dialysis membrane when the dialysis chamber was drained. Autoradiography showed this DNA to be several hundred microns long, indicating a molecular weight of 109 or more. Short fragments, which would correspond to the usual molecular weight of extracted bacterial DNA, were not seen.

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