Interaction of 6-Thiopurines and Thiol-Containing RNA with a Cellulose Mercurial

Tipo de material: Texto

TextoSeries ; Analytical Biochemistry, 92(2), p.324-330, 1979Trabajos contenidos:

Melvin, W. T
Keir, H.M

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Resumen: Thiol-containing bases such as 6-mercaptopurine bind to mercurated cellulose under conditions where non-thiol bases do not bind. Both RNA and thiol-containing RNA bind to mercurated cellulose, but the affinity for thiol-containing RNA is considerably greater. Ligands such as CN- and S,O,'- inhibit this binding to mercurated cellulose, and conditions can be found which permit almost complete retention. of thiol-containing RNA with low retention of non-thiol-containing RNA. A simple method is described for separating newly synthesised RNA containing thiol groups from older RNA which does not contain thiol groups.

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Thiol-containing bases such as 6-mercaptopurine bind to mercurated cellulose under conditions where non-thiol bases do not bind. Both RNA and thiol-containing RNA bind to mercurated cellulose, but the affinity for thiol-containing RNA is considerably greater. Ligands such as CN- and S,O,'- inhibit this binding to mercurated cellulose, and conditions can be found which permit almost complete retention. of thiol-containing RNA with low retention of non-thiol-containing RNA. A simple method is described for separating newly synthesised RNA containing thiol groups from older RNA which does not contain thiol groups.

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