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Protoplast Transformation of Filamentous Fungi

Tipo de material: TextoTextoSeries ; Molecular and Cell Biology Methods for Fungi, Methods in Molecular Biology, 638, p.3-19, 2010Trabajos contenidos:
  • Turgeon, B.G
  • Condon, B
  • Liu, J
  • Zhang, N
Tema(s): Recursos en línea: Resumen: The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI)procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-12992 (Browse shelf(Opens below)) Available

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI)procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

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