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The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis

Tipo de material: TextoTextoSeries ; Physiol. Plant., 43, p.104-106, 1978Trabajos contenidos:
  • Fridborg, G
  • Pedersen, M
  • Landstrom, L
  • Eriksson, T
Recursos en línea: Resumen: Cell suspensions of Daucus carota and Haplopappus graciiis and callus suspensions of Allum cepa var. proliferum were grown in media with and without activated charcoal. Differentiation occurred in those Daucus and A Ilium cultures that contained charcoal. It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and /i-OH-benzoic acid {Daucus), 2,6-OH-benzoic acid {Allium)and benzoic acid, pelargonic acid and caprylic acid {Haplopappus), whereas the media with activated charcoal did not. It was also shown that p- OH-benzoic acid had inhibitory effects on the embryogenesis in Daucus cultures.
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Cell suspensions of Daucus carota and Haplopappus graciiis and callus suspensions of Allum cepa var. proliferum were grown in media with and without activated charcoal. Differentiation occurred in those Daucus and A Ilium cultures that contained charcoal. It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and /i-OH-benzoic acid {Daucus), 2,6-OH-benzoic acid {Allium)and benzoic acid, pelargonic acid and caprylic acid {Haplopappus), whereas the media with activated charcoal did not. It was also shown that p- OH-benzoic acid had inhibitory effects on the embryogenesis in Daucus cultures.

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