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Studies on nitrogen metabolism during somatic embryogenesis in carrot. I. Utilization of α-alanine as a nitrogen source

Tipo de material: TextoTextoSeries ; Plant Science Letters, 33, p.7-13, 1984Trabajos contenidos:
  • Kamada, H
  • Harada, H
Tema(s): Recursos en línea: Resumen: a-Alanine added to a culture medium was incorporated into cells and/or embryos during rapid cell proliferation and globular embryo formation, in which an active protein synthesis was occurring. After the incorporation into cells, a-alanine seemed to be quickly transformed to glutamic acid by alanine aminotransferase and utilized as a nitrogen source. Alanine aminotransferase activity was observed in cells and/or embryos, although the activity decreased during culture period. Utilization of a-alanine by cultured cells as a nitrogen source was discussed in relation to its stimulative effect on somatic embryogenesis.
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a-Alanine added to a culture medium was incorporated into cells and/or embryos during rapid cell proliferation and globular embryo formation, in which an active protein synthesis was occurring. After the incorporation into cells, a-alanine seemed to be quickly transformed to glutamic acid by alanine aminotransferase and utilized as a nitrogen source. Alanine aminotransferase activity was observed in cells and/or embryos, although the activity decreased during culture period. Utilization of a-alanine by cultured cells as a nitrogen source was discussed in relation to its stimulative effect on somatic embryogenesis.

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