Molecular cloning and organ-specific expression of three isoforms of tomato ADP-glucose pyrophosphorylase gene

We isolated three cDNAs encoding different isoforms of ADP-glucose pyrophosphorylase (AGP)large submits from tomato plants. Three clones, designated AgpL1, AgpL2, and AgpL3 were 2019, 2105, and 1850 bp, respectively. The clones had a long, uninterrupted open reading frame with a start codon at the 5¾ region and different copies of polyadenylation signal (AATAAA)at the 3¾ region, deriving 57-58 kDa polypeptides. Sequence comparison and phylogenetic analysis revealed that the three isoforms represented different types of AGP large subunits, AgpL1 was strongly expressed in stems and weakly in roots. Accumulation of AgpL1 transcripts was found even in unpollinated ovaries and sustained at the early stages of fruit development. ApgL2 was expressed in roots and fruits. AgpL3 was exclusively expressed in leaves. The present study suggests that the three isoforms of tomato AGP large subunits are organ-specific in their expressions.