Protoplasts isolated from callus cells of maize endosperm. Formation of multinucleate protoplasts and nuclear division

Tipo de material: Texto

TextoSeries ; Exprl Cell Res, 68(2), p.452-456, 1971Trabajos contenidos:

Motoyoshi, F

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Resumen: About 50 per cent of the total protoplasts enzymatically isolated from callus cells of maize endosperm were multinucleate, suggesting that fusion of single protoplasts occurred during the protoplast isolation process. Some of the protoplasts cultured in vitro showed enlargement, budding, and nuclear division during 6 days of culture. Nuclear division in the multinucleate protoplasts was usually synchronous. No evidence of protoplast proliferation has yet been obtained.

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About 50 per cent of the total protoplasts enzymatically isolated from callus cells of maize endosperm were multinucleate, suggesting that fusion of single protoplasts occurred during the protoplast isolation process. Some of the protoplasts cultured in vitro showed enlargement, budding, and nuclear division during 6 days of culture. Nuclear division in the multinucleate protoplasts was usually synchronous. No evidence of protoplast proliferation has yet been obtained.

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