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IN VITRO CULTURE OF ALOE BARBADENSIS Mill.: MORPHOGENETIC ABILITY AND NUCLEAR DNA CONTENT

Tipo de material: TextoTextoSeries ; Plant Science, 55(1), p.53-59, 1988Trabajos contenidos:
  • Castorena Sanchez, I
  • Natali, L
  • Cavallini, A
Tema(s): Recursos en línea: Resumen: In vitro culture of Aloe barbadensis is very difficult for both callus induction and plant regeneration. By contrast, micropropagation from shoot meristems is easily achieved. A DNA microdensitometric study was performed on different organs of A. barbadensis and during in vitro culture of different explants. It was shown that morphogenetic ability is correlated to nuclear DNA content in the explant and/or in the callus. In particular, plant regeneration (or micropropagation)is obtained only from caUi (or from explants)in which the amount of DNA per nucleus falls strictly within the diploid limits (2C and 4C)of the species.
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In vitro culture of Aloe barbadensis is very difficult for both callus induction and plant regeneration. By contrast, micropropagation from shoot meristems is easily achieved. A DNA microdensitometric study was performed on different organs of A. barbadensis and during in vitro culture of different explants. It was shown that morphogenetic ability is correlated to nuclear DNA content in the explant and/or in the callus. In particular, plant regeneration (or micropropagation)is obtained only from caUi (or from explants)in which the amount of DNA per nucleus falls strictly within the diploid limits (2C and 4C)of the species.

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