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Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana : Method 3

Tipo de material: TextoTextoSeries ; Methods in Molecular Biology, 990, p.109-118, 2013Trabajos contenidos:
  • Xiaohui Yang
  • Li Yuan
  • Makaroff, C.A
Tema(s): Recursos en línea: Resumen: Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana . This chapter describes fi xation, sample preparation, and immunolocalization techniques used in our laboratory. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation.
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Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana . This chapter describes fi xation, sample preparation, and immunolocalization techniques used in our laboratory. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation.

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