A versatile transformation system for the cellulolytic filamentous fungus Triclroderma reesei

Tipo de material: Texto

TextoSeries ; Gene, 61(2), p.155-164, 1987Trabajos contenidos:

Penttilsl, M
Nevalainen, H
Ratto, M
Salminen, E
Knowles, J

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Resumen: An eficient transformation system for the cellulolytic tilamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per pg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80

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An eficient transformation system for the cellulolytic tilamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per pg of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80

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