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Asexual Embryogenesis via Callus of Theobroma cacao 1

Tipo de material: TextoTextoSeries ; Z. Pflanzenphysiol., 13, p.347-358., 1984Trabajos contenidos:
  • Kononowicz, H
  • Kononowicz, A.K
  • Janick, J
Tema(s): Recursos en línea: Resumen: Callus of Theobroma cacao L. (cacao)possessing embryogenic competence occurred spontaneously with two clones of asexual embryos proliferated in vitro in a hormone-free basal medium by hypocotylary budding. Asexual embryogenesis via callus occurred at low frequency in the hormone-free basal medium. High concentrations of 2,4-D plus coconut water stimulated callus production and suppressed embryo production. Maximum frequency and intensity of embryogenesis occurred at 10-3 to 10-2 mg ·12,4-D. Embryos originated from meristematic tissue at the periphery of callus clumps. During development asexual embryos either remained embedded in the callus or were connected through suspensor-like structures.
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Callus of Theobroma cacao L. (cacao)possessing embryogenic competence occurred spontaneously with two clones of asexual embryos proliferated in vitro in a hormone-free basal medium by hypocotylary budding. Asexual embryogenesis via callus occurred at low frequency in the hormone-free basal medium. High concentrations of 2,4-D plus coconut water stimulated callus production and suppressed embryo production. Maximum frequency and intensity of embryogenesis occurred at 10-3 to 10-2 mg ·12,4-D. Embryos originated from meristematic tissue at the periphery of callus clumps. During development asexual embryos either remained embedded in the callus or were connected through suspensor-like structures.

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