Cytokinin Mediated Long-term, High-frequency Plant Regeneration in Rice Tissue Cultures

Tipo de material: Texto

TextoSeries ; Z. Pflanzenphysiol., 113, p.315-323, 1984Trabajos contenidos:

Raghava Ram, N. V
Nabors, M. W

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Resumen: In Mahsuri rice callus cultures grown in light, the total amount of callus and the percentage of callus volume composed of embryogenic cells could be increased if 0.1-0.Smg·I-1 K was added to medium containing 0.5 or 1.0 mg .1-1 2,4-D. Embryogenic regions could be proliferated and maintained on the same media. E callus on initiation medium gradually produced embryoid-like structures and finally plantlets. This process could be hastened by removing E callus to a regeneration medium containing low concentrations of IAA and BAP or K. If the E callus produced by one seed were proliferated for five four-week passages on 0.5 mg .1-1 2,4-D and 0.5 mg .1-1 K and transferred to regeneration medium, 7,318 plants could be produced from 184 g of E callus.

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In Mahsuri rice callus cultures grown in light, the total amount of callus and the percentage of callus volume composed of embryogenic cells could be increased if 0.1-0.Smg·I-1 K was added to medium containing 0.5 or 1.0 mg .1-1 2,4-D. Embryogenic regions could be proliferated and maintained on the same media. E callus on initiation medium gradually produced embryoid-like structures and finally plantlets. This process could be hastened by removing E callus to a regeneration medium containing low concentrations of IAA and BAP or K. If the E callus produced by one seed were proliferated for five four-week passages on 0.5 mg .1-1 2,4-D and 0.5 mg .1-1 K and transferred to regeneration medium, 7,318 plants could be produced from 184 g of E callus.

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