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Evaluation of Nosema ceranae spore-specific polyclonal antibodies

Tipo de material: TextoTextoSeries ; Journal of Apicultural Research, 50(2), p.145-151, 2011Trabajos contenidos:
  • Aronstein, K.A
  • Saldivar, E
  • Webster, T.C
Tema(s): Recursos en línea: Resumen: A new genomic antibody (Ab)has been developed against a spore-wall protein SWP-32 of the honey bee intracellular pathogen, Nosema ceranae. In dot blots and Western blots this Ab specifically recognized N. ceranae spore antigens and did not cross-react with N. apis spore lysates, unless blots were overdeveloped. The detection sensitivity depends on both the concentration of the anti-SWP-32 Ab and the concentration of Nosema spores in the lysates. To avoid non-specific staining, we suggest using this new Ab at 1:5000 dilution for detection of 1 × 103 and higher spore numbers per assay. Considering that a single infected bee can produce over 50 × 106 spores, this level of sensitivity will allow detection of a very low level of Nosema infection in bee colonies.
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A new genomic antibody (Ab)has been developed against a spore-wall protein SWP-32 of the honey bee intracellular pathogen, Nosema ceranae. In dot blots and Western blots this Ab specifically recognized N. ceranae spore antigens and did not cross-react with N. apis spore lysates, unless blots were overdeveloped. The detection sensitivity depends on both the concentration of the anti-SWP-32 Ab and the concentration of Nosema spores in the lysates. To avoid non-specific staining, we suggest using this new Ab at 1:5000 dilution for detection of 1 × 103 and higher spore numbers per assay. Considering that a single infected bee can produce over 50 × 106 spores, this level of sensitivity will allow detection of a very low level of Nosema infection in bee colonies.

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