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Bixin and norbixin in human plasma: Determination and study of the absorption of a single-dose of annatto food color

Tipo de material: TextoTextoSeries ; Analyst, 122(9), p.977-980, 1997Trabajos contenidos:
  • Levy, L.W
  • Regalado, E
  • Navarrete, S
  • Watkins, R.H
Tema(s): Recursos en línea: Resumen: A procedure was developed for the detection and determination of bixin and norbixin in human plasma by reversed-phase HPLC with a sensitivity limit of 5 µg l-1. A group of seven volunteers ingested a single dose of 1 ml of a commercial Annatto Food Color (16 mg of cis-bixin in soybean oil). The presence of bixin (cis and trans)and norbixin (cis and trans)was demonstrated in the plasma at average levels of 11.6, 10.1, 2.8 and 0 µg l-1 of bixin and 48, 58, 53 and 29 µg l-1 of norbixin after 2, 4, 6 and 8 h, respectively. Considerable individual variations were observed. Complete plasma clearance generally occurred for bixin by 8 h and for norbixin by 24 h after ingestion of cis-bixin.
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A procedure was developed for the detection and determination of bixin and norbixin in human plasma by reversed-phase HPLC with a sensitivity limit of 5 µg l-1. A group of seven volunteers ingested a single dose of 1 ml of a commercial Annatto Food Color (16 mg of cis-bixin in soybean oil). The presence of bixin (cis and trans)and norbixin (cis and trans)was demonstrated in the plasma at average levels of 11.6, 10.1, 2.8 and 0 µg l-1 of bixin and 48, 58, 53 and 29 µg l-1 of norbixin after 2, 4, 6 and 8 h, respectively. Considerable individual variations were observed. Complete plasma clearance generally occurred for bixin by 8 h and for norbixin by 24 h after ingestion of cis-bixin.

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