Genome Editing to Improve Abiotic Stress Responses in Plants

Tipo de material: Texto

TextoSeries ; Progress in Molecular Biology and Translational Science, 149, p.99-109, 2017Trabajos contenidos:

Osakabe, Y
Osakabe, K

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Resumen: Targeted modification of specific genes via genome editing is now used routinely to modify plant genomes. In developing new mutations in plant genomes using the widely used CRISPR/Cas9 system, it is important for further use in plant molecular studies and crop breeding that the mutations generated are heritable. To date, several improvements to increase efficiency and specificity have been developed to generate heritable mutations in various plant species. In this chapter, we focus on strategies to improve genome editing technology to increase heritability in plants, and summarize the process used to generate new mutant alleles of environmental stress response genes in plants. Such studies suggest further applications in molecular breeding to improve plant function using optimized plant CRISPR/Cas9 systems.

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Previous	B-1678 In Vitro Propagation of Grape	B-16780 On Improving CRISPR for Editing Plant Genes: Ribozyme-Mediated Guide RNA Production and Fluorescence-Based Technology for Isolating Transgene-Free Mutants Generated by CRISPR	B-16781 CRISPR/Cas9-Enabled Multiplex Genome Editing and Its Application	B-16782 Genome Editing to Improve Abiotic Stress Responses in Plants	B-16783 Use of CRISPR/Cas9 for Symbiotic Nitrogen Fixation Research in Legumes	B-16784 Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus	B-16785 CRISPR/Cas9-Based Genome Editing in Plants	Next

Targeted modification of specific genes via genome editing is now used routinely to modify plant genomes. In developing new mutations in plant genomes using the widely used CRISPR/Cas9 system, it is important for further use in plant molecular studies and crop breeding that the mutations generated are heritable. To date, several improvements to increase efficiency and specificity have been developed to generate heritable mutations in various plant species. In this chapter, we focus on strategies to improve genome editing technology to increase heritability in plants, and summarize the process used to generate new mutant alleles of environmental stress response genes in plants. Such studies suggest further applications in molecular breeding to improve plant function using optimized plant CRISPR/Cas9 systems.

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