Quantitative analysis of cytokinins by electrospray tandem mass spectrometry

Tipo de material: Texto

TextoSeries ; Rapid Communications in Mass Spectrometry, 9(10), p.948-953, 1995Trabajos contenidos:

Prinsen, E
Redig, P
Van Onckelen, H.A
Van Dongen, W
Esmans, E.L

Tema(s):

MASS SPECTROMETRY

Recursos en línea:

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Resumen: A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin-riboside injected on-column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1-100 pmol injected on-column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution. This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.

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A methodology was developed for the analysis of cytokinins from plant tissue using electrospray tandem mass spectrometry in combination with HPLC. A detection limit of 1 pmol zeatin-riboside injected on-column was achieved under multiple reaction monitoring conditions. Linearity was observed within a concentration range of 1-100 pmol injected on-column. In view of the analysis of a considerable amount of samples, the chromatographic conditions were selected which were a compromise between speed of analysis and resolution. This approach, in combination with multiple reaction monitoring, allowed the analysis of up to 150 samples a day, and quantitation of 16 different cytokinins per sample.

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