Differentiating races of Corynespora cassiicola using RAPD and internal transcribed spacer markers

Tipo de material: Texto

TextoSeries ; J Rubb Res, 6, p.58-64, 2003Trabajos contenidos:

Atan, S

Tema(s):

CORYNESPORA CASSIICOLA

Recursos en línea:

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Resumen: Nine isolates of Corynespora cassiicola from Hevea brasiliensis were analysed using two molecular marker techniques: random amplified polymorphic DNA (RAPD); and the amplification of the internal transcribed spacer (ITS)regions of ribosomal DNA. RAPD distinguished two groups of isolates i.e. isolates infectious to RRIM 2020 clones, and isolates that infected RRIM 600 and several other clones of H. brasiliensis. The ITS region of eight out of nine isolates were, found to be monomorphic.

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Nine isolates of Corynespora cassiicola from Hevea brasiliensis were analysed using two molecular marker techniques: random amplified polymorphic DNA (RAPD); and the amplification of the internal transcribed spacer (ITS)regions of ribosomal DNA. RAPD distinguished two groups of isolates i.e. isolates infectious to RRIM 2020 clones, and isolates that infected RRIM 600 and several other clones of H. brasiliensis. The ITS region of eight out of nine isolates were, found to be monomorphic.

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