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A Comparative Study of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)versus SCX-IMAC-Based Methods for Phosphopeptide Isolation/Enrichment

Tipo de material: TextoTextoSeries ; Journal of Proteome Research , 7, p.4869-487, 2008Trabajos contenidos:
  • Gan C. S
  • Guo T
  • Zhang H
  • Kiang S
Tema(s): Recursos en línea: Resumen: Lectrostatic repulsion-hydrophilic interaction chromatography (ERLIC)has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311)than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12percent unique phosphopeptides were common to both pproaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCXIMAC with ERLIC
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Lectrostatic repulsion-hydrophilic interaction chromatography (ERLIC)has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311)than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12percent unique phosphopeptides were common to both pproaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCXIMAC with ERLIC

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