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Domestication of wild tomato is accelerated by genome editing

Tipo de material: TextoTextoSeries ; Nature Biotechnology, 36(12), p.1160-1164, 2018Trabajos contenidos:
  • Li T
  • Yang X
  • Yu Y
  • Si X
  • Zhai X
  • Zhang H
  • Dong W
  • Dong W
  • Xu C
Tema(s): Recursos en línea: Resumen: Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR-Cas9 editing of coding sequences, cisregulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.
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Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR-Cas9 editing of coding sequences, cisregulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.

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